microarray bead plate Search Results


dna template  (Thermo Fisher)
99
Thermo Fisher dna template
A schematic representation of the RAPID-M technology. The modified T7Term primer was mixed with the immobilization beads in <t>a</t> <t>PCR</t> microwell plate ( a ) to create beads-immobilized T7Term primer ( b ). The target <t>DNA</t> is directly immobilized to the beads by PCR amplification using beads-immobilized T7Term and soluble <t>T7Prom</t> to create high density immobilized DNA (HDID) in each well ( c ). The beads-immobilized DNA is translated to protein with the addition of cell-free reagents ( d , e ) and the protein is arrayed onto Ni–NTA slides using a microarray printer to produce high density protein microarray slides (HDS) ( f )
Dna Template, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+bead+plate/DNA/pmc04642736-62-6-41
Average 99 stars, based on 1 article reviews
dna template - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier

Image Search Results


A schematic representation of the RAPID-M technology. The modified T7Term primer was mixed with the immobilization beads in a PCR microwell plate ( a ) to create beads-immobilized T7Term primer ( b ). The target DNA is directly immobilized to the beads by PCR amplification using beads-immobilized T7Term and soluble T7Prom to create high density immobilized DNA (HDID) in each well ( c ). The beads-immobilized DNA is translated to protein with the addition of cell-free reagents ( d , e ) and the protein is arrayed onto Ni–NTA slides using a microarray printer to produce high density protein microarray slides (HDS) ( f )

Journal: BMC Research Notes

Article Title: Development of repeatable arrays of proteins using immobilized DNA microplate (RAPID-M) technology

doi: 10.1186/s13104-015-1637-3

Figure Lengend Snippet: A schematic representation of the RAPID-M technology. The modified T7Term primer was mixed with the immobilization beads in a PCR microwell plate ( a ) to create beads-immobilized T7Term primer ( b ). The target DNA is directly immobilized to the beads by PCR amplification using beads-immobilized T7Term and soluble T7Prom to create high density immobilized DNA (HDID) in each well ( c ). The beads-immobilized DNA is translated to protein with the addition of cell-free reagents ( d , e ) and the protein is arrayed onto Ni–NTA slides using a microarray printer to produce high density protein microarray slides (HDS) ( f )

Article Snippet: Standard PCR conditions were: 100–150 ng DNA template (pIVEX2.3d-GFP or ompA linearized template, 1 mM MgSO 4 , 1X PCR buffer, 0.3 mM dNTP mix, 0.8 pmol T7Prom soluble primer, 5 μg beads-immobilized primer and 1.25 U Platinum pfx DNA polymerase (Invitrogen) in a final reaction volume of 10 μl.

Techniques: Modification, Amplification, Microarray

Western blot analysis of GFP ( a ) and OmpA ( b ) proteins using beads-immobilized DNA template. Proteins were detected using peroxidase-conjugated anti-polyHistidine monoclonal antibody. The calculated protein sizes for GFP and OmpA are 30 and 27 kDa, respectively. DNA templates used for cell-free expression were as follows: a Lane 1 , gfp -SA beads template; lane 2 , gfp -CA beads template; lane 3 , soluble DNA coding for GFP (as positive control); lane 4 , no DNA template (as negative control). b Lane 1 , no DNA template (as negative control); lane 2 , ompA -CA beads template; lane 3 , ompA -SA beads template; lane 4 , soluble ompA protein coding DNA (as positive control)

Journal: BMC Research Notes

Article Title: Development of repeatable arrays of proteins using immobilized DNA microplate (RAPID-M) technology

doi: 10.1186/s13104-015-1637-3

Figure Lengend Snippet: Western blot analysis of GFP ( a ) and OmpA ( b ) proteins using beads-immobilized DNA template. Proteins were detected using peroxidase-conjugated anti-polyHistidine monoclonal antibody. The calculated protein sizes for GFP and OmpA are 30 and 27 kDa, respectively. DNA templates used for cell-free expression were as follows: a Lane 1 , gfp -SA beads template; lane 2 , gfp -CA beads template; lane 3 , soluble DNA coding for GFP (as positive control); lane 4 , no DNA template (as negative control). b Lane 1 , no DNA template (as negative control); lane 2 , ompA -CA beads template; lane 3 , ompA -SA beads template; lane 4 , soluble ompA protein coding DNA (as positive control)

Article Snippet: Standard PCR conditions were: 100–150 ng DNA template (pIVEX2.3d-GFP or ompA linearized template, 1 mM MgSO 4 , 1X PCR buffer, 0.3 mM dNTP mix, 0.8 pmol T7Prom soluble primer, 5 μg beads-immobilized primer and 1.25 U Platinum pfx DNA polymerase (Invitrogen) in a final reaction volume of 10 μl.

Techniques: Western Blot, Expressing, Positive Control, Negative Control

Repetitive cell-free expression of GFP and OmpA proteins using immobilized DNA templates. a Lane 1 , GFP from soluble DNA (as positive control); lanes 2–5 , GFP protein from the first, second, third and fourth sequential rounds of cell-free expression using gfp -CA beads template; lanes 6-9 , GFP protein from the first, second, third and fourth sequential rounds of cell-free expression using gfp -SA beads template; lane 10 , no DNA template (as negative control). b Lanes 1–4 , OmpA protein from the first, second, third and fourth sequential rounds of cell-free expression using ompA -CA beads template; lane 5 , OmpA protein from soluble DNA (as positive control); lane 6 , no DNA template (as negative control)

Journal: BMC Research Notes

Article Title: Development of repeatable arrays of proteins using immobilized DNA microplate (RAPID-M) technology

doi: 10.1186/s13104-015-1637-3

Figure Lengend Snippet: Repetitive cell-free expression of GFP and OmpA proteins using immobilized DNA templates. a Lane 1 , GFP from soluble DNA (as positive control); lanes 2–5 , GFP protein from the first, second, third and fourth sequential rounds of cell-free expression using gfp -CA beads template; lanes 6-9 , GFP protein from the first, second, third and fourth sequential rounds of cell-free expression using gfp -SA beads template; lane 10 , no DNA template (as negative control). b Lanes 1–4 , OmpA protein from the first, second, third and fourth sequential rounds of cell-free expression using ompA -CA beads template; lane 5 , OmpA protein from soluble DNA (as positive control); lane 6 , no DNA template (as negative control)

Article Snippet: Standard PCR conditions were: 100–150 ng DNA template (pIVEX2.3d-GFP or ompA linearized template, 1 mM MgSO 4 , 1X PCR buffer, 0.3 mM dNTP mix, 0.8 pmol T7Prom soluble primer, 5 μg beads-immobilized primer and 1.25 U Platinum pfx DNA polymerase (Invitrogen) in a final reaction volume of 10 μl.

Techniques: Expressing, Positive Control, Negative Control

Application of the RAPID-M method for generation of protein arrays. The proteins were printed using the Nano-Plotter™ NP 2.1. Immobilized proteins were in situ purified on Ni–NTA chip and subjected to detection using peroxidase-conjugated anti-polyHistidine antibody followed by addition of TSA-Cy5. Lane 1–4 , proteins from repetitive cycles of first, second, third and fourth rounds of cell-free expression using immobilized DNA template. Proteins are printed in 3 × 3 blocks and each block represents one cycle of cell-free expression using beads-immobilized DNA

Journal: BMC Research Notes

Article Title: Development of repeatable arrays of proteins using immobilized DNA microplate (RAPID-M) technology

doi: 10.1186/s13104-015-1637-3

Figure Lengend Snippet: Application of the RAPID-M method for generation of protein arrays. The proteins were printed using the Nano-Plotter™ NP 2.1. Immobilized proteins were in situ purified on Ni–NTA chip and subjected to detection using peroxidase-conjugated anti-polyHistidine antibody followed by addition of TSA-Cy5. Lane 1–4 , proteins from repetitive cycles of first, second, third and fourth rounds of cell-free expression using immobilized DNA template. Proteins are printed in 3 × 3 blocks and each block represents one cycle of cell-free expression using beads-immobilized DNA

Article Snippet: Standard PCR conditions were: 100–150 ng DNA template (pIVEX2.3d-GFP or ompA linearized template, 1 mM MgSO 4 , 1X PCR buffer, 0.3 mM dNTP mix, 0.8 pmol T7Prom soluble primer, 5 μg beads-immobilized primer and 1.25 U Platinum pfx DNA polymerase (Invitrogen) in a final reaction volume of 10 μl.

Techniques: In Situ, Purification, Expressing, Blocking Assay